Int J Biochem Mol Biol 2013;4(4):209-214
Original Article
Gene environment interaction in preterm delivery with special reference to
organochlorine pesticide: a case control study
Tusha Sharma, Basu Dev Banerjee, Md Mustafa, Kiran Guleria, Rafat S Ahmed, Ashok K Tripathi
Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry, Department of Obstetrics and Gynaecology,
University College of Medical Sciences & G.T.B. Hospital (University of Delhi), Dilshad Garden, Delhi 110 095, India
Received November 13, 2013; Accepted November 28, 2013; Epub December 15, 2013; Published December 30, 2013
Abstract: Objectives: To assess the Gene-Environmental interaction between maternal organochlorine pesticides (OCPs) level and CYP17
gene polymorphism with the risk of preterm delivery (PTD). Materials and methods: Maternal blood samples of hundred cases (n = 100) of PTD
and of equal number of healthy controls were collected at the time of delivery. OCPs levels were estimated by Gas chromatography system
equipped with electron capture detector and PCR-RFLP was used for polymorphic analysis of CYP17 gene. Results: Significantly (p < 0.05)
higher levels of α-HCH, β-HCH, and γ-HCH were found in maternal blood samples of PTD cases as compared to controls. We did not found
any significant difference in the frequency genotype distribution CYP17 gene in PTD cases as compared to controls. When gene environmental
interaction between the CYP17 gene polymorphism and OCPs level was considered, a significant interaction was observed between ≥ 50th
percentile of γ-HCH and CYP17 A1A1 (wild type) genotype. Conclusions: Higher levels of OCPs along with wild type state of CYP17 gene
(A1A1) in women may be considered as an important etiological factor in ‘idiopathic’ PTD. The present study provides evidence that genetic
variation and its interaction with the environmental exposure may increase the risk of PTD. (IJBMB1310004).
Keywords: Preterm delivery, organochlorine pesticides, CYP17 gene polymorphism, gene-environment interaction
Address correspondence to: Dr. Basu Dev Banerjee, Environmental Biochemistry and Molecular Biology Laboratory, Department of
Biochemistry, University College of Medical Sciences & G.T.B. Hospital, University of Delhi, Dilshad Garden, Delhi 110 095, India. Tel: +91-11-
22135362; Fax: +91-11-22590495; E-mail: banerjeebd@hotmail.com
Original Article
Gene environment interaction in preterm delivery with special reference to
organochlorine pesticide: a case control study
Tusha Sharma, Basu Dev Banerjee, Md Mustafa, Kiran Guleria, Rafat S Ahmed, Ashok K Tripathi
Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry, Department of Obstetrics and Gynaecology,
University College of Medical Sciences & G.T.B. Hospital (University of Delhi), Dilshad Garden, Delhi 110 095, India
Received November 13, 2013; Accepted November 28, 2013; Epub December 15, 2013; Published December 30, 2013
Abstract: Objectives: To assess the Gene-Environmental interaction between maternal organochlorine pesticides (OCPs) level and CYP17
gene polymorphism with the risk of preterm delivery (PTD). Materials and methods: Maternal blood samples of hundred cases (n = 100) of PTD
and of equal number of healthy controls were collected at the time of delivery. OCPs levels were estimated by Gas chromatography system
equipped with electron capture detector and PCR-RFLP was used for polymorphic analysis of CYP17 gene. Results: Significantly (p < 0.05)
higher levels of α-HCH, β-HCH, and γ-HCH were found in maternal blood samples of PTD cases as compared to controls. We did not found
any significant difference in the frequency genotype distribution CYP17 gene in PTD cases as compared to controls. When gene environmental
interaction between the CYP17 gene polymorphism and OCPs level was considered, a significant interaction was observed between ≥ 50th
percentile of γ-HCH and CYP17 A1A1 (wild type) genotype. Conclusions: Higher levels of OCPs along with wild type state of CYP17 gene
(A1A1) in women may be considered as an important etiological factor in ‘idiopathic’ PTD. The present study provides evidence that genetic
variation and its interaction with the environmental exposure may increase the risk of PTD. (IJBMB1310004).
Keywords: Preterm delivery, organochlorine pesticides, CYP17 gene polymorphism, gene-environment interaction
Address correspondence to: Dr. Basu Dev Banerjee, Environmental Biochemistry and Molecular Biology Laboratory, Department of
Biochemistry, University College of Medical Sciences & G.T.B. Hospital, University of Delhi, Dilshad Garden, Delhi 110 095, India. Tel: +91-11-
22135362; Fax: +91-11-22590495; E-mail: banerjeebd@hotmail.com
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